Nanoscale probing the kinetics of oriented bacterial cell growth using atomic force microscopy.

Ultra structural characteristics of methicillin resistant Staphylococcus aureus cell wall after affecting with lytic bacteriophages using atomic force microscopy

Objective(s): During the last years with increasing resistant bacteria to the most antibiotics bacteriophages are suggested as appropriate treatment option. To investigate lytic activity of bacteriophages there are indirect microbial procedures and direct methods. The present study to complement microbial procedures and investigate ultra-structural characteristics of infection bacterium-phage u...

Force nanoscopy of living cells

The emerging field of ‘live cell nanoscopy’ has revolutionized the way biologists explore the living cell at molecular resolution. Whereas far-field fluorescence nanoscopy enables the study of the nanoscale localization and dynamics of biomolecules in cells, recent developments in atomic force microscopy (AFM) techniques offer unprecedented opportunities for imaging the structural heterogeneity...

Probing attractive forces at the nanoscale using higher-harmonic dynamic force microscopy

Probing Nanoscale Heat and Force Interactions Using Atomic Force Microscopes (afm)

Many devices and instruments such as magnetic hard disk drives and atomic force microscopes (AFM) rely on the stable operation of their small probing heads at nanoscale gaps. Due to the small scale of the probing heads, the force interactions (Casimir force and electrostatic force) between the small probes and the surrounding become more significant. The local heating caused by read/write elect...

Bacterial adhesion force quantification by fluidic force microscopy.

Quantification of detachment forces between bacteria and substrates facilitates the understanding of the bacterial adhesion process that affects cell physiology and survival. Here, we present a method that allows for serial, single bacterial cell force spectroscopy by combining the force control of atomic force microscopy with microfluidics. Reversible bacterial cell immobilization under physio...